José Manuel Pérez Pérez Group

Responsible José Manuel Pérez Pérez
Website http://arolab.edu.umh.es/

 

RESEARCH SUMMARY

Cellular dedifferentiation, the process by which a differentiated somatic cell reverts to an undifferentiated state, is a key process during tissue regeneration after wounding, in both plants and animals. Besides transcription factors, epigenetic factors also contribute to transcriptional reprogramming by creating an active or silent chromatin configuration; some of which are essential for maintaining stem cell activity.

arolab portada

The young root system of tomato plants is mostly composed of adventitious roots emerging form the hypocotyl, also known as shoot-borne roots.

 

RESEARCH LINES

 

Line 1: Genetic control of cellular reprogramming during de novo organ formation in plants

We have established a model for wound-induced adventitious root (wiAR) formation in tomato hypocotyls where specific auxin-responsive genes activate cambial cell division and root primordia specification in a subset of vascular cells. By combining genetic and biochemical approaches, we will identify the gene regulatory networks involved in wiAR formation and their conservation in other regenerative pathways.

arolab línea 1

Transverse section of vascular bundle showing initiation of a wiAR primordium. Scale bar: 100 µm.

 

Line 2: Identification of key factors involved in root system architecture (RSA) in cultivated tomato and wild relatives

Manipulation of RSA traits may improve water and nutrient capture under normal and extreme climate conditions. By using a genetical genomics approach, our group aims to elucidate the role of new regulators affecting valuable RSA traits.

arolab línea 2

Manhattan plot for the number of wiARs obtained from a GWAS (Justamante et al., 2019).

 

Nombre Puesto Correo ORCID
José Manuel Pérez Pérez Full Professor 0000-0003-2848-4919
Taras Pasternak Postdoctoral researcher “María Zambrano” 0000-0001-5061-4076
Eduardo Larriba Tornel Postdoctoral researcher 0000-0001-8838-4974
María Salud Justamante Clemente Postdoctoral researcher 0000-0002-3291-2800
Riyazuddin Riyazuddin PhD Student 0000-0003-2580-2811
Olha Yaroshko PhD Student 0000-0003-2517-4472
Carlos Torralbo Torrado PhD Student  
Lara Caballero López Laboratory Technician (“Investigo GVA”)  
Adrián Luque Torres Research Technician  
María José Ñíguez Gómez Laboratory Technician SATDI  

 

grupo jmperez_2021

 

 

Pasternak, K. Palme, J.M. Pérez-Pérez
Role of reactive oxygen species in the modulation of auxin flux and root development in Arabidopsis thaliana
Plant Journal 114: 83-95 (2023)

I. Friero, E. Larriba, P.A. Martínez-Melgarejo, M.S. Justamante, M.V. Alarcón, A. Albacete, J. Salguero, J.M. Pérez-Pérez
Transcriptomic and hormonal analysis of the roots of maize seedlings grown hydroponically at low temperature
Plant Science 326: 111525 (2023)

Larriba, A.B. Sánchez-García, M.S. Justamante, C. Martínez-Andújar, A. Albacete, J.M. Pérez-Pérez
Dynamic hormone gradients regulate wound-induced de novo organ formation in tomato hypocotyl explants
International Journal of Molecular Sciences. 22: 11843 (2021).

Alaguero-Cordovilla, A.B. Sánchez-García, S. Ibáñez, A. Albacete, A. Cano, M. Acosta, J.M. Pérez-Pérez
An auxin‐mediated regulatory framework for wound‐induced adventitious root formation in tomato shoot explants
Plant Cell and Environment 44: 1642-1662 (2021).

Mhimdi, J. M. Pérez-Pérez
Understanding of adventitious root formation: what can we learn from comparative genetics?
Frontiers in Plant Science 11: 582020 (2020).

Alaguero-Cordovilla, F.J. Gran-Gómez, P. Jadczak, M. Mhimdi, S. Ibáñez, C. Bres, D. Just, C. Rothan, J.M. Pérez-Pérez
A quick protocol for the identification and characterization of early growth mutants in tomato
Plant Science 301: 110673 (2020).

Ibáñez, H. Ruiz-Cano, M.Á. Fernández, A.B. Sánchez-García, J. Villanova, J.L. Micol, J.M. Pérez-Pérez
A network-guided genetic approach to identify novel regulators of adventitious root formation in Arabidopsis thaliana
Frontiers in Plant Science 10: 461 (2019).

M.S. Justamante, S. Ibáñez, A. Peidró, J.M. Pérez-Pérez
A genome-wide association study identifies new loci required for wound-induced lateral root formation in Arabidopsis thaliana
Frontiers in Plant Science 10: 311 (2019).

Cano, A.B. Sánchez-García, A. Albacete, R. González-Bayón, M.S Justamante, S. Ibáñez, M. Acosta, J.M. Pérez-Pérez
Enhanced conjugation of auxin by GH3 enzymes leads to poor adventitious rooting in carnation stem cuttings
Frontiers in Plant Science 9: 566 (2018).

E. Bustillo-Avendaño, S. Ibáñez, O. Sanz, J.A.S. Barros, I. Gude, J. Perianez-Rodriguez, J.L. Micol, J.C. Del Pozo, M.A. Moreno-Risueño, J.M. Pérez-Pérez
Regulation of hormonal control, cell reprograming and patterning during de novo root organogenesis
Plant Physiology 176: 1709-1727 (2018).

Genome wide association studies of root system architecture traits associated with water and nutrient use efficiency in tomato (TED2021-132256B-C22). Agencia de financiación: Ministerio de Ciencia e Innovación. Vigencia 2022 – 2024
IP: José Manuel Pérez Pérez

Unveiling the role of the plant LSD1 complex during adventitious root formation (PID2021-126840OB-I00). Agencia de financiación: Ministerio de Ciencia e Innovación. Vigencia 2022 – 2024
IP: José Manuel Pérez Pérez

Transfer of ancestral traits from the root system to cultivated tomato varieties to improve water- and nutrient-use efficiency (AGROALNEXT/2022/036). Agencia de financiación: Conselleria d’Innovació, Universitats, Ciència i Societat Digital. Vigencia 2022 – 2024
IP: José Manuel Pérez Pérez

Genetic reprogramming of cell pluripotency in differentiated plant tissues (GRISOLIAP/2019/098). Agencia de financiación: Conselleria d’Educació, Cultura i Esport. Vigencia: 2019 – 2022.
IP: José Manuel Pérez Pérez

Light sheet microscopy unit for comparative genomics of adventitious root development in tomato and carnation (IDIFEDER/2018/016). Agencia de financiación: Conselleria d’Educació, Cultura i Esport. Vigencia: 2018 – 2020.
IP: José Manuel Pérez Pérez

Transfer of ancestral traits from the root system to cultivated tomato varieties to improve water- and nutrient-use efficiency (AGROALNEXT/2022/036). Agencia de financiación: Conselleria d’Innovació, Universitats, Ciència i Societat Digital. Vigencia 2022 – 2024
IP: José Manuel Pérez Pérez

Genetic reprogramming of cell pluripotency in differentiated plant tissues (GRISOLIAP/2019/098). Agencia de financiación: Conselleria d’Educació, Cultura i Esport. Vigencia: 2019 – 2022.
IP: José Manuel Pérez Pérez

Light sheet microscopy unit for comparative genomics of adventitious root development in tomato and carnation (IDIFEDER/2018/016). Agencia de financiación: Conselleria d’Educació, Cultura i Esport. Vigencia: 2018 – 2020.
IP: José Manuel Pérez Pérez

Tesis recientes

  • Sergio Ibáñez López (2023). Nuevas funciones génicas en la formación de raíces adventicias en Arabidopsis.
  • Virginia Birlanga Murcia (2022). Caracterización de los factores abióticos que intervienen en la aparición de ciertas fisiopatías en el cultivo sin suelo
  • Aurora Alaguero Cordovilla (2022). Caracterización morfológica de la arquitectura radicular en plántulas de tomate y su regulación hormonal.
  • María Salud Justamante Clemente (2020). Estudio de los mecanismos fisiológicos y moleculares implicados en la formación y desarrollo de raíces adventicias en distintas especies vegetales
    [PDF].
  • Joan Villanova Calatayud (2017). Desarrollo de herramientas para la mejora genética del enraizamiento de esquejes en clavel cultivado (Dianthus caryophyllus L.).

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